|Name||Ferritin RIA Test kit|
|Full name||Human Ferritin RIA Test kit|
|Category Name||Anemia RIA kits|
|Detection Range||0-2000 ng/mL|
|Shelf Life||45 Months from the manufacturing date|
Ferritin RIA kit description:
This Ferritin RIA test kit is a Quantitative test based on Immunoradiometric assay that screens for Iron-deficiency anemia.
Materials provided with Ferritin RIA Test Kit:
1. Ferritin Monoclonal antibody coated tubes (100 tubes)
2. FERRITIN monoclonal antibody Labelled with I 125 (Tracer I125)
3. Ferritin test reference standard set, contains 0, 5, 20, 50,250, 500, 1000 & 2000 ng/ml, ready for use
4. Diluent Solution : BSA – PBS
5. Control serum: ferritin in serum with preservatives(1Vial, 1 ml)
Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. Gama counter
Ferritin molecule consists of 24 peptide subunits with molecular weight of 20,000 for each subunit. Ferritin serves to store iron in body tissues in a non-toxic form, to deposit it in a safe form for the cells. Ferritin is available in liver, spleen and bone marrow with a high concentration. In the shell of each Ferritin molecule there are about 3000 to 4500 of iron atoms (Fe3+) that contains approximately 20% of whole body iron. There is a little Ferritin in the serum with direct relationship with the stored Ferritin in body tissues; so, measurement of the available Ferritin in the serum is used to determination of whole body iron store. Ferritin serum concentration level is related to body iron stores and is influenced by several changes in the amount of the iron due to age and gender. The influence of factors has a significant impact on iron balance. Measurement of the serum Ferritin is important for high and low concentration of the iron diagnosis and diagnosis of iron deficiency anemia with other anemia. Newborns have high cord serum ferritin levels that decrease in childhood period but a progressive increase in male adults causing the excess of iron storage in the body and increase of the Ferritin. Ferritin level decreases in pregnant women until the menopause period but then Ferritin level of the serum increases in females. High serum Ferritin levels associate with Hereditary hemochromatosis and iron overload. Ferritin moderate increase can be seen in acute inflammations caused by infection, injury or tumor and liver diseases.
Principle of the assay
Immune radiometric assay (IRMA) kit, evaluates FERR based on the one step non-competitive reaction. In this type of assay, the method employs two highly specific monoclonal anti-FERR antibodies which recognize two different epitopes of the molecule. One antibody is coated on solid phase (coated tube), the other, specific for the FERR and labelled with Iodine-125, is used as a tracer. Both of these antibodies react with FERR antigens which are present in standard, control serum and the sample. Unspecific materials are removed in the washing step. The amount of activity form in the tube has a direct relationship to the FERR concentration. FERR standards with known concentrations are tested with the unknown samples in which the concentration of unknown samples is achieved based on the standard curve of the counted amount against concentration of FERR.