| Name | Methadone Urine Rapid Test (Strip/cassette) |
| Category Name | Drug Tests |
| Test | 25 or 50 tests |
| Cut-Off | 300 ng/ml |
The MTD Rapid Test (Urine) is a lateral flow chromatographic immunoassay for the detection of Methadone in human urine at a cut-off concentration of 300ng/ml.
Materials Provided with MTD drug Rapid Test kit:
1. MTD Test Device
The test contains mouse monoclonal anti-Methadone antibody coupled particles and Methadone-protein conjugate. A goat antibody is employed in the control line system.
2. Instructions for use
Materials required, not Provided:
1. Urine collection container
2. Timer or clock
MTD Rapid Test kit Background Information
Methadone is a narcotic pain reliever for medium to severe pain. It is also used in the treatment of Heroin (Opiate dependence: Vicodin, Percolate, Morphine, etc) addiction. Oral Methadone is very different than the IV Methadone. Oral Methadone is partially stored in the liver for later use. IV Methadone acts more like Heroin.
Methadone is a long acting pain reliever producing effects that last between twelve to forty-eight hours. Ideally, Methadone frees the client from the pressures of obtaining illegal Heroin, from the dangers of injection, and from the emotional roller coaster that most Opiates produce. Methadone, if taken for long periods and at large doses, can lead to a very long withdrawal period. The withdrawals from Methadone are more prolonged and troublesome than those provoked by heroin cessation, yet the substitution and phased removal of methadone is an acceptable method of detoxification for patients and therapists.
The MTD Rapid Test (Urine) is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Methadone in urine. The MTD Rapid Test (Urine) yields a positive result when the Methadone in urine exceeds 300 ng/mL.
MTD drug Rapid Test Principle
The MTD Rapid Test (Urine) is an immunoassay based on the principle of competitive binding. Drugs that may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary action. Methadone, if present in the urine specimen below 300 ng/mL, will not saturate the binding sites of antibody-coated particles in the test. The antibody coated particles will then be captured by immobilized Methadone-protein conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Methadone level exceeds 300 ng/mL because it will saturate all the binding sites of anti-Methadone antibodies.
A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration less than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.