|Name||PSA RIA kit|
|Category Name||Cancer RIA kits|
|Detection Range||0-100 ng/mL|
|Sample||25 µl serum|
|Total Time||~80 min|
|Shelf Life||45 Months from the manufacturing date|
PSA RIA kit description:
PSA RIA test kit is an Immunoradiometric assay which provides a quantitative measurement of PSA in human serum.
Materials provided with PSA RIA Test Kit:
1. Coated Tubes: Anti –PSA monoclonal Antibody (100 tubes)
2. Anti-PSA monoclonal antibody Labelled with I 125 (Tracer I125)
3. PSA test reference standard set, contains 0, 0.8, 2.5, 5,15, 50, 100 ng/ml, ready for use
4. Serum Diluent : BSA – PBS
5. Control serum: PSA in serum with preservatives(1Vial, 0.5 ml)
6. Washing Solution (Concentrted): Tween 20solution
Materials Required, not Provided:
1. Precision pipettes
2. Gama counter
Prostate-specific antigen (PSA) is a 34-kDa glycoprotein with serine protease activity. PSA is a prostate tissue-specific antigen in terms of immunological view and is secreted by normal cells, benign and cancerous cells of prostate epithelial. PSA is separated from prostatic acid phosphatase due to immunochemical functions. PSA concentrations increase in serum of patients with prostate cancer, benign prostatic hypertrophy and inflammatory conditions associated with surgery and metastasis. Currently, PSA is known as a valuable serum marker with a high accuracy in diagnosis and treatment monitoring of prostate cancer by surgery or other known therapies.
Principle of the assay
Immune radiometric assay (IRMA) kit, evaluates PSA based on the one step non-competitive reaction. In this type of assay, the method employs two highly specific monoclonal anti- PSA antibodies which recognize two different epitopes of the molecule. One antibody is coated on solid phase (coated tube), the other, specific for the PSA and labelled with Iodine-125, is used as a tracer. Both of these antibodies react with PSA antigens which are present in standard, control serum and the sample. Unspecific materials are removed in the washing step. The amount of activity form in the tube has a direct relationship to the PSA concentration. PSA standards with known concentrations are tested with the unknown samples in which the concentration of unknown samples is achieved based on the standard curve of the counted amount against concentration of PSA.