| Name | Cocaine Urine Rapid Test (Strip/cassette) |
| Category Name | Drug Tests |
| Test | 25 or 50 tests |
| Cut.Off | 300 ng/ml |
The COC Rapid Test is a rapid chromatographic immunoassay for the qualitative detection of Cocaine metabolite, Benzoylecgonine, in human urine at a cut-off concentration of 300 ng/mL.
Materials Provided with COC drug Rapid Test kit:
1. COC Test Device
The test contains mouse monoclonal anti-Benzoylecgonine antibody-coupled particles and Benzoylecgonine-protein conjugate. A goat antibody is employed in the control line system.
2. Instructions for use
Materials required, not Provided:
1. Urine collection container
2. Timer or clock
COC Rapid Test kit Background Information
Cocaine is a potent central nervous system (CNS) stimulant and a local anesthetic. Initially, it brings about extreme energy and restlessness while gradually resulting in tremors, over-sensitivity and spasms. In large amounts, Cocaine causes fever, unresponsiveness, and difficulty in breathing and unconsciousness. Cocaine is often self-administered by nasal inhalation, intravenous injection and free-base smoking. It is excreted in the urine in a short time primarily as Benzoylecgonine.1,2 Benzoylecgonine, a major metabolite of Cocaine, has a longer biological half -life (5 - 8 hours) than Cocaine (0.5 - 1.5 hours), and can generally be detected for 24-48 hours after Cocaine exposure. The COC (Cocaine) Test is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Cocaine metabolite in urine. The COC Rapid Test yields a positive result when the Cocaine metabolite in urine exceeds 300ng/ml.
COC drug Rapid Test Principle
The COC Rapid Test is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody. During testing, a urine specimen migrates upward by capillary action. Benzoylecgonine, if present in the urine specimen below 300ng/ml, will not saturate the binding sites of antibody in the test. The antibody coated particles will then be captured by immobilized Benzoylecgonine conjugate and a visible colored line will appear in the test line region. The colored line will not form in the test line region if the Benzoylecgonine level is above 300ng/ml because it will saturate all the binding sites of antibodies. A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration less than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.